How Do I Create An Experimental Design Testing The Effectiveness Of Two Different Antibiotics?
Uncategorized December 3rd, 2009Here’s the actual question:
You are a microbiologist who has been hired by a drug company to determine if their new drug, “bacteriakill,” is more effective than penicillin. Design an experiment to test the effectiveness of the two antibiotics. Your experimental design must include:
a) a purpose
b) appropriate safety precautions (aseptic techniques)
c) an easy-to-follow, detailed procedure, written in third person
d) an observation table that could be used to record data
Help??????
December 3rd, 2009 at 6:44 am
Use antibiotic sensitivity assay (Kirby-Bauer method)
Take two different petriplates inoculate both the plates with a strain of bacteria which you wish to test
In one plate place a disc of penicillin, in another place a disc of “bacteriakill” in the centre of plate
Incubate plate for 24 hours
Observe the zone of inhibition (clear area of no bacterial growth) diameter
The one that shows bigger ZOI is the better antibiotic